RESULTS
We took measurements on three different days. These days were each three days apart. PAR = photosynthetically
available light
In the interval between the first and second time measurements were taken, there was an increase in both the cell counts and the concentration of chlorophyll a. Graph lines from both graphs show about the same slope. However, the clustering that occurred when measurements were taken a second time do not show any significant differences between the three treatments (PAR, PAR + UVA, and PAR + UVA + UVB). Possible explanations for this may be that the weather during this time was cloudy and overcast. This might have resulted in lower ultraviolet radiation. It was also noted that condensation occurred on the top of the boxes and that could have interfered with transmittance of ultraviolet radiation into the cultures.
A few cautions should be noted. The bags were floating in different positions and in different places in the boxes causing uneven exposure to sunlight. Also it is important to start with new materials on the box lids because over time mylar deteriorates. In conclusion, both cell counts and chlorophyll a concentration exhibit similar trends over time. Therefore, both or either could be used to measure the growth of phytoplankton cultures. |
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The Woodrow Wilson National Fellowship Foundation CN 5281, Princeton NJ 08543-5281 - Tel:(609)452-7007 - Fax:(609)452-0066 Technical contact: lpt@woodrow.org |