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PCR Time Warp


 


DNA Learning Center: PCR Animation
http://www.geneticorigins.org/geneticorigins/pv92/aluframeset.htm

Once you are at this website, you will have to select the ‘Media and Animations’ button at the top of the page.  On the next page, select the ‘PCR Animation’ under the ‘Theory’ heading.  If you don’t have Macromedia’s Shockwave or Flash Player you will have to download this software from the link the DNA Learning Center provides.  It is well worth it.

PCR Animation by Robert J. Husky – University of Virginia
http://www.people.virginia.edu/~rjh9u/pcranim.html

This animation provides a very limited description of the process and proceeds through 4 cycles of the process.

PCR Animation by M.A. Campbell – Davison College, North Carolina
http://www.bio.davidson.edu/courses/Immunology/Flash/RT_PCR.html

This animation is interactive and contains some details regarding the chemical components involved in the reaction.  The student must click a next button to continue through the steps of the animation.

Oak Ridge National Laboratories – PCR Animation
http://www.ornl.gov/hgmis/publicat/twistedladder/pcrdemo.html

This animation is well done, runs on its own if Quicktime is installed, and has audio and textual descriptions of the process.

PCR Animation by M.K. Raghuraman at the University of Washington

http://depts.washington.edu/~genetics/courses/genet371b-aut99/PCR_contents.html

This animation focuses on the three steps: denaturing, annealing, and extending and has information at the level of single nucleotides.  This page also contains an animation on ‘choosing primers’.

PCR Animation by Andy Vierstraete – University of Ghent
http://allserv.rug.ac.be/~avierstr/principles/pcrani.html

This animation focuses on the three steps of PCR:  denaturing, annealing, and extending and has a nifty thermometer in the animation showing changes in the temperature.  This page also contains a high quality link on the ‘principles of PCR’.

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