WWLPT

"Fishing for Microsatellites"|

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Microsatellite 

Back Simulation
 

1. Have the parental strands (two red strands) pair up again while the other students remain in wait.
    

2. Have them simulate DNA replication using only the existing 5’ to 3’ strand and the students having the complimentary green sequences.  One student with the repeat CAAT will not be able to participate at this time.
    

3. After they do so have the red student strand creates a slip strand mispairing (see below).  At this point the green student strand should be one student too long and you should simulate the enzymatic excision of one of the repeat student sequences.  You should also simulate the enzymatic excision of the slip strand student (see below).  Now you should have a double strand that has one fewer microsatellite repeat.  Review how this occurred and lead them to the mechanism discussed in the article. 

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