Gene CleaningGene Cleaning
Procedure Page
This process cleans DNA after PCR. The leftover dNTPs, leftover enzymes, and leftover PCR primers need to be removed from the copies of DNA segments.
1. Transfer PCR results to a 1.5 μl tube.
2. Add 75 μl of Sodium Iodide (NaI) from a Q-bio-gene kit.
3. Add 7 μl of glass milk. This is a mixture of microscopic glass beads suspended in water. Double stranded DNA sticks to the beads. Glass milk may need to be vortexed or pipetted to resuspend it.
4. Centrifuge to pellet the glass beads to the bottom.
5. Remove the supernatant liquid from the tube.
6. Add 500 μl of New Wash and resuspend.
7. Centrifuge to pellet the glass beads to the bottom.
8. Remove the supernatant liquid from the tube.
9. Add 20 μl sterile distilled water and resuspend.
10. Incubate the tubes in a 65 C water bath for at least 10 minutes (or up to an hour). This will unstick the DNA from the beads.