The handlers: Jenifer,
Jessica, Jeffrey, Evelyn and Phil
Here's How to .........
. . . . . . Use a Plastic Pipette
Pipettes are used as a tool for transferring fluids.
Pipettes can be graduated for measurement of small volumes of material.
Squeeze the bulb before inserting the tip of the
pipette into the liquids of choice.
Insert the pipette into the liquid to be moved.
Release your grip on the bulb. The liquid will be
sucked into the pipette.
Move your pipette to the next container and squeeze
the bulb. Its contents will be pushed into the container.
*Be cautious about sucking particulate matter into
the shaft of the pipette. The tip of the pipette can be enlarged if
required by cutting the end.
. . . . . Use a Plastic
- Insert the
cotton-filled end of a sterile pipette into the end of a plastic pipette
- Both pipettes
and pumps come in different sizes. Be sure to use a pump that can
"suck" the quantity of liquid you want (e.g. 10 ml pump with a 5 ml or
10 ml sterile pipette).
- Using the wheel at
the top of the pump, raise the white top of the pump approximately 1 cm
BEFORE inserting the pipette into the liquid.
- This step is
necessary in order to create additional air pressure in the pipette as
you expel the liquid.
- Next, place the
tapered tip of the pipette into the liquid. The tip should be within
the liquid during the pipetting.
- Hold the pipette
pump with one hand--your thumb should be placed on the wheel.
- Use your thumb to
rotate the wheel downward. This will cause the liquid to rise into the
pipette. Do this carefully and watch the meniscus of the liquid rise to
your desired level.
Measure the level of the liquid at the BOTTOM of the meniscus.
- Next, take the tip
of the pipette out of the liquid and move the entire apparatus to the
place where you desire to put the measured liquid.
thumb to rotate the wheel upward. This will cause the liquid to be
dispensed from the pipette. Lower the white top of the pump all the way
to the pump shaft. Because you created the extra space before pipetting,
the entire contents of the pipette should be now be dispensed!
. . . . . Use a Pipette with a Bulb
There are 3 areas on the bulb that can be
squeezed: A (air), S (suck) and E (empty). Identify these areas and give
them a quick squeeze just to check it out.
Put a pipette into the bulb, under area S.
Insert the cotton-filled end carefully.
Always hold the pipette with the open end
down to prevent solutions from entering the bulb.
Be aware that there are different
graduations on different sizes of pipette. Make sure you use the correct
Squeeze the bulb while holding area A.
This creates a vacuum.
Put the end of the pipette tube into the
liquid, and squeeze area S. Watch carefully, the liquid rises quickly.
Fill the pipette to a level just higher than you need for the experiment.
Release area S.
Put the pipette tube into the area you want
to dispense the liquid. Squeeze area E to release the fluid. The harder
you squeeze, the faster the liquid comes out.
Dispose of pipette properly when finished.
. . . . . Use a Micropipette
The micropipette is
used to measure a very small and accurate volume of fluid
Micropipettes can be
found in varieties that have set, unalterable fluid volumes or in
varieties with various adjustable fluid ranges.
Select the appropriate pipette for the desired volume
The range of the pipette can be found on the top of
the pipette plunger.
Use the correct tip
for the specific micropipette size.
Adjust the volume of
the micropipette by turning the dial to the desired volume needed.
Depress the plunger
to the first level for the intake of the fluid.
Place the tip of the
micropipette in the desired fluid, and slowly release pressure, allowing
the plunger to rise, thus allowing for the intake of the fluid.
Transfer the fluid to
its desired location by depressing the plunger to its first position.
Second click the plunger to allow for the full transfer of the fluid.
Remove the tip from
the pipette, with the plunger down, by pressing on the eject button.
Always hold the point of the
barrel down at all times to prevent fluids from entering the pump
Always dispense used tips between each transfer.
Do not stick micropipette tips in ears, eyes, nose or
mouth or other openings in the body.