Modifications for K-8 for each experiment are listed below
The Contact Slide
Carbon Dioxide Evolution
Winogradsky Columns



The Contact Slide

Materials:
containers or plastic two liter soda pop bottles - with the top two or three inches cut off
microscope slides (need to be glass, not plastic)
balance or another type of scale for weighing in grams
chemicals (consult a table of chemicals in household products to find a source for these chemicals)
staining rack ( paper towels on which to lay slides to dry after they have been stained)
water bath (plastic bottle of water with narrow spout for slowly squirting water on slides)
microscope (magnifying glasses will allow students to see colonies but not individual bacteria)

Procedures:
1. 1:1 = amount of compost equals amount of sandy loam
2. Label the glossy part of each slide as control 1, 2, 3, treatment 1-1, 1-2, 1-3, treatment 2-1, 2-2, 2-3, or use some other system to identify each slide and from which jar each came.
If students can not do this without difficulty, either use small spatula, or craft stick to poke hole before sliding in the slide, or do it for students
3. Be sure to label the contents of each treatment
4. Do not wash slides under running water, as you will lose microbes
Top of page

Carbon Dioxide Evolution

Materials:
amendments: consult a table of chemicals in household products to find a source for these chemicals
burettes: ignore this equipment - not important

Procedures:
label jars as control, treatment 1, treatment 2, with changes made
Top of page



Winogradsky Modifications K-8

Purpose: This can also be used as an introduction to density.

Materials:
children's sand and beach toys (buckets and shovels) work well for collecting sample
glass or plexiglas column: liter and two liter soda pop bottles may also be used
calcium sulfate
sodium sulfate
calcium carbonate
consult a table of chemicals in household products to find a source of these chemicals
two liter soda pop bottles with about two or three inches cut off the top can be used for mixing
spoons will also be needed for mixing

Procedures:
1. Write name of student with date at bottom of column container if tape is needed to write student's name and date, make sure it is in a place on the column that will not hide any substance in column.

2. The first time this is done, you may want all students to add materials by layer the second time, students can choose what to put in a mixing container, stir it up, removing sticks, rocks, etc. that are too large, then pour into their column container each day the students can observe how their column is changing and how it compares to their layered column.

3 & 4.
grams of sediment
grams of calcium sulfate
grams of calcium carbonate
and sodium sulfate
100
0.5
0.25
150
0.75
0.375
200
1.00
0.5
etc.

5. Whichever amendments are going to be added to sediment for the layered column, need to be added before sediment is put into column with the mixed, or second, column, the amendments may be added at any time before mixture is poured into column.

6. Column may also be covered with aluminum foil, etc. to slow evaporation rate with or without covering, water may need to be added from time to time to keep column at same level as it started.

Top of page
back to microbial succession page


b
The Woodrow Wilson National Fellowship Foundation
CN 5281, Princeton NJ 08543-5281 - Tel:(609)452-7007 - Fax:(609)452-0066
Technical contact: lpt@woodrow.org