NITRATE LOADING OF STREAMS
Agriculture vs.  Suburban vs. Recreation
A New Jersey Study
A Woodrow Wilson Environmental Science
Leadership Institute
Summer 1997
The Earth and It's Systems

 
    This study represents a collaborative effort of four team members:
    Nancy Vawter & Annie G. Wells, Alabama State University,
    Montgomery, Alabama, and Helen W. Ballard & Gloria
    Stallworth, Fairfield City Schools, Fairfield, Alabama.  We
    represent teams 25 and 33.
 
 

This project  is on-going, with applications continued with nitrate loaded waters in our  home state of Alabama.  We will train teachers on the testing of the stream, collection and recording of data, who in turn will teach their students to collect this data in local streams.

BACKGROUND INFORMATION

    *What is "nitrate loading" of streams?
    *How is it caused?
    *What is the effect on the biosphere?
    *What factors may reduce the contamination?
Nitrate loading of streams is the response to ground water runoff from fertilized lawns of homes, golf courses, and agricultural fields. All across the country, particularly New Jersey, Maryland, and New York, pollution is killing  fish and other aquatic life; destroying acres of wetlands and contaminating both surface water and  groundwater, (water table) with chemicals that are potentially dangerous to human health.

The source of these contaminants include everything from livestock manure, fertilizers, and  mining metals to suburban lawn chemicals. When rain water washes over land, it carries these contaminants into streams or drains down into the ground water.  By the time these contaminants reach the streams, they are in  excess and thereby "loads" the stream in a disproportionate ratio to the amount of water.

For example, excessive nitrates from fertilizers cause algal blooms, these blooms in turn deplete the oxygen supply in lakes and streams. In turn the fish die as a result of a lack of oxygen.  Growth studies demonstrated that when nitrates are added to streams, certain species of algae increase in quantity (Rhine, Crump & Jordan 1985).
 
 HYPOTHESIS
If  stream contamination is related to land use, then the runoff from agricultural land is greater than that of suburbia and recreation.
 
INTRODUCTION
Among the areas of concern within the realm of environmental quality as it relates to human survivability, is water pollution.  Despite innovative programs created by the amendments to the 1987 Clean Water Act, toxic runoff remains the nation's most deadly water dilemma.
 
MATERIALS
      topographical map of East Windsor- Highstown area
      vehicle for transporting to and from sites
      HACH Aqua Culture Testing Kits
      *pH meter-stream specific probe
      *portable spec
      *D O test kit and chemicals
      *turbidity kits
      FS Nitrate Testing Kit
 The procedure listed by the company for each test was followed and an explanation of each type of  test is referenced below under background on test  performed.

TESTS
     pH
     dissolved oxygen
     temperature
     turbidity
     nitrates

WHY THESE TESTS?

 pH
A pH of 7 is considered neutral.  A pH of 9 and above is considered high. Studies on the production of algae show that the higher the pH,  (9-10); the lower the contamination;  lower contamination = low growth rate of algae. pH is important to this study due to the fact that nitrification occurs most readily at a pH of 5.5 to 6.5.

DISSOLVED OXYGEN
Ammonia converts to nitrate when highly aerated.  Less dissolved oxygen, results in more nitrate loading; more nitrate loading leads to less oxygen, with less oxygen more fish will die.

TEMPERATURE/DISSOLVED OXYGEN
As the temperature of the water rises, the rate of photosynthesis increases,  therefore not only do more plants grow, more plants die.  When a plant dies, the bacteria which decomposes them require oxygen,  Therefore, an increase in the rate of photosynthesis is equal  to an increase in the need for oxygen, AND a decrease in the amount of dissolved oxygen.

TURBIDITY
 Turbidity is a measure of how clear the water is.  The more murky the water, the higher the turbidity.  Urban runoff is one of several causes of high turbidity. The higher the turbidity, the higher the temperature and the less diversity of aquatic organisms.

NITRATE
The higher the nitrate level in streams, the lower the aquatic life.
 

PROCEDURE
Using topographical maps of Princeton specific to Hightstown, sample sites were chosen.
 

Our sites included water sources involving Lake Etra, Peddie Lake/ Rocky Brook at two locations in the East Windsor-Hightstown area.  At each test site it was difficult to find  one which was totally agricultural, suburban or recreational.

   Agricultural-Lake Etra-- surrounded by corn, soybean and various row crops
                                       on the east end. Eutrophication has resulted in
                                       formation of a semi wetland.  Small community of
                                       houses are on the southeast end.
 
   Recreational- Peddie Lake/Rocky Brook-flows from Lake Etra through a
                                       small neighborhood, but mainly represents the runoff
                                       from a golf course which is one-fourth mile from
                                       the site tested.

   Suburbia-  Rocky Brook, Bend Road location- surrounded by homes and a
                                       small public park.  Samples were tested at the Bank
                                       Street location to aquire the runoff from the suburban
                                        homes.  Water here is free flowing with a small
                                        number of ducks.
 
The investigators researched the rainfall in the area and determined that there had been negligible rainfall in the area consisting of a few scattered showers with little to no accumulation of  rain for the water table.

Visually we noted that the corn in the area was beginning to curl due to the heat and lack of rain.  The earth appeared dry and in some places cracked. According to local sources there had not been any negligible rainfall in the area for approximately two months.

The stream waters were low in the Rocky Brook- Bank Street location. There was no rainfall for a 38 hour period prior to the time the first samples were tested. Tests were conducted on samples from approximately 10 a.m. through late afternoon.  The second samples were tested a little less than 16 hours after a severe thunderstorm.  Field lab tests were the format. Dissolved oxygen tests were not performed the first day because of lack of chemicals to test.
 

 
                                  Lake Etra showing eutrophication to a new wetland
 

 RESULTS FROM THE SITES WHICH DRAIN FROM Etra LAKE INTO ROCKY BROOK.

                                                   NITRATES    TEMPERATURE
  SITES                   D.O              (p.p.m.)            degrees C              pH                TURBIDITY
BendRd/Rocky 
Br   Suburbia 1
       n/a   2.5 27.5 7.64 low-<1
BendRd/Rocky Br  Suburia 2        .15   2.5 25.0 7.2 low-<1
agr-Lake Etra1         n/a    .5 25.6 7.13 low-<1
agr-Lake Etra-2         0.1    .5 20.4 6.5 low-<1
Rec- Golf C 1         n/a  2.5 27.7 8.9 low-<1
Rec-Golf C-2         .25  2.5 25.0 7.2 low-<1
The test kits used were  the FS Nitrate Testing Kit, the HACH portable Aqua Culture Kit, pH/temp
 meter/probe and the portable  HACH DO kit.
 *n/a=not availiable
 
Data Interpretation
The indication of our data is that the agricultural streams show less nitrate loading than the golf course and the suburb.  This aspect of the data ia also verified contrasting the samples tested for lowest averages of  pH, and temperature. It should be noted that the location tested for agricultural runoff was done in a lake which showed eutrophication due to the long and continuous nutrient feeding from agricultural runoff.  More test in an agricultural area (not fed by wetlands) would have to be done in order to prove the hypothesis.

 In an interview with a road worker, (cutting the grass alongside the road for the telephone company), herbicides and pesticides are not used, but roadsides are commonly mowed.   Another interview with a bridge builder netted the same response.  Other people interviewed substantiated these responses. A sewage plant operator told investigators that lime and chlorine are added at
3 p.p.m. at a constant injection rate and that the only other chemicals used at the treatment plant is an injection of fluoride.  We concluded that no other chemicals were responsible for the condition of the waters around Hightstown.
 
HYPOTHESIS  REJECTED 
 If stream contamination is related to land use, then the runoff from agricultural land is not any greater than that of suburban or recreational.
 
Contamination of Study
In an effort to provide clear data investigators submit the following for scrutiny. The following is a list of items which could/may have contributed to contamination of our data.

     1. lack of samples taken,
     2. lack of acquired samples done at more interval sites along the Millstone Watershed,
     3. lack of dissolved oxygen test done on day one,
     4. time of dissolved oxygen done on second day, and
     5. depth of water from which sample was taken.
 
 
 

 SCIENCE CONTENT STANDARDS FOR THE NEW JERSEY STUDY
 
     Science as Inquiry

     CONTENT STANDARD A:
     As a result of activities in grades 5-8, all students should develop

          Abilities necessary to do scientific inquiry

          Understandings about scientific inquiry
 
DEVELOPING STUDENT ABILITIES AND UNDERSTANDING
Students in grades 5-8 should be provided opportunities to engage in full and in partial  inquiries. In a full inquiry students begin with a question, design an investigation, gathers evidence, formulate an answer to the original question, and communicates the investigative  process and results. In partial inquiries, they develop abilities and understanding of selected aspects of the inquiry process. Students might, for instance, describe how they would design an invention
Students in grades 5-8 can begin to recognize the relationship between explanation and
evidence. They can understand that background knowledge and theories guide the design of investigations, the types of observations made, and the interpretations of data. In turn, the
experiments and investigations students conduct become experiences that shape and modify their background knowledge.

Several factors of this standard should be highlighted. The instructional activities of a scientific
 inquiry should engage students in identifying and shaping an understanding of the question under inquiry. Students should know what the question is asking, what background knowledge is being used to frame the question, and what they will have to do to answer the question. The students' questions should be relevant and meaningful for them. To help focus investigations, students should frame questions, such as "What do we want to find out about . "Nitrate Loading of Streams in Alabama as Compared to New Jersey".
 
Science Content Standards for 9-12
Science Content Standard A

Identify Questions and Concepts that Guide Scientific Investigations
Students should formulate a testable hypothesis and demonstrate the logical connections between the scientific concepts guiding a hypothesis and the design of an experiment.  They should demonstrate appropriate procedures, a knowledge base, and conceptual understanding of scientific investigations.
Design and Conduct Scientific Investigations
Designing and conducting a scientific investigation requires introduction to the major concepts in the area being investigated proper equipment, safety precautions, assistance with methodological problems, recommendations for use of technologies, clarification of ideas that guide the inquiry, and scientific knowledge obtained from sources other than the actual investigation.  The investigation may also require student clarification of the question, method, controls, and variables; student organization and display of data; student revision of methods and explanations; and a public presentation of the results with a critical response from peers.  Regardless of the scientific investigation performed, students must use evidence, apply logic, and construct an argument for their propose explanations.
 USE TECHNOLOGY AND MATHEMATICS TO IMPROVE INVESTIGATIONS
     AND COMMUNICATIONS. A variety of technologies, such as hand tools, measuring
     instruments, and calculators, should be an integral component of scientific investigations. The
     use of computers for the collection, analysis, and display of data is also a part of this standard.
     Mathematics plays an essential role in all aspects of an inquiry. For example, measurement is
     used for posing questions, formulas are used for developing explanations, and charts and
     graphs are used for communicating results.

     FORMULATE AND REVISE SCIENTIFIC EXPLANATIONS AND MODELS
     USING LOGIC AND EVIDENCE. Student inquiries should culminate in formulating an
     explanation or model. Models should be physical, conceptual, and mathematical. In the
     process of answering the questions, the students should engage in discussions and arguments
     that result in the revision of their explanations. These discussions should be based on scientific
     knowledge, the use of logic, and evidence from their investigation.

     RECOGNIZE AND ANALYZE ALTERNATIVE EXPLANATIONS AND
     MODELS. This aspect of the standard emphasizes the critical abilities of analyzing an
     argument by reviewing current scientific understanding, weighing the evidence, and examining
     the logic so as to decide which explanations and models are best. In other words, although
     there may be several plausible explanations, they do not all have equal weight. Students
     should be able to use scientific criteria to find the preferred explanations.

     COMMUNICATE AND DEFEND A SCIENTIFIC ARGUMENT. Students in school
     science programs should develop the abilities associated with accurate and effective
     communication. These include writing and following procedures, expressing concepts,
     reviewing information, summarizing data, using language appropriately, developing diagrams
     and charts, explaining statistical analysis, speaking clearly and logically, constructing a
     reasoned argument, and responding appropriately to critical comments.

     USE TECHNOLOGY AND MATHEMATICS TO IMPROVE INVESTIGATIONS
     AND COMMUNICATIONS. A variety of technologies, such as hand tools, measuring
     instruments, and calculators, should be an integral component of scientific investigations. The
     use of computers for the collection, analysis, and display of data is also a part of this standard.
     Mathematics plays an essential role in all aspects of an inquiry. For example, measurement is
     used for posing questions, formulas are used for developing explanations, and charts and
     graphs are used for communicating results.

     FORMULATE AND REVISE SCIENTIFIC EXPLANATIONS AND MODELS
     USING LOGIC AND EVIDENCE. Student inquiries should culminate in formulating an
     explanation or model. Models should be physical, conceptual, and mathematical. In the
     process of answering the questions, the students should engage in discussions and arguments
     that result in the revision of their explanations. These discussions should be based on scientific
     knowledge, the use of logic, and evidence from their investigation.

     RECOGNIZE AND ANALYZE ALTERNATIVE EXPLANATIONS AND
     MODELS. This aspect of the standard emphasizes the critical abilities of analyzing an
     argument by reviewing current scientific understanding, weighing the evidence, and examining
     the logic so as to decide which explanations and models are best. In other words, although
     there may be several plausible explanations, they do not all have equal weight. Students
     should be able to use scientific criteria to find the preferred explanations.

     COMMUNICATE AND DEFEND A SCIENTIFIC ARGUMENT. Students in school
     science programs should develop the abilities associated with accurate and effective
     communication. These include writing and following procedures, expressing concepts,
     reviewing information, summarizing data, using language appropriately, developing diagrams
     and charts, explaining statistical analysis, speaking clearly and logically, constructing a
     reasoned argument, and responding appropriately to critical comments.

Physical Science

Content Standard B:  As a result of their activities in grades 9-12, all students should develop an understanding of:
Chemical reactions
Interactions of energy and matter

Environmental Quality
Natural ecosystems provide an array of basic processes that affect humans.  Those processes include maintenance of the quality of the atmosphere, generations of soils, control of the hydrologic cycle, disposal of wastes, and recycling of nutrients.  Humans are chancing many of these basic processes, and the changes may be detrimental to humans.  See Content Standard C (grades 9-12)

Materials from human societies affect both physical and chemical cycles of the earth.

Many factors influence environmental quality.  Factors that students might investigate include population growth, resource use, population distribution, over consumption, the capacity of technology to solve problems, poverty, the role of economic, political, and religious views, and different ways humans view the earth.
 
 

     Science and Technology

     CONTENT STANDARD E: As a result of activities in grades 9-12, all students
     should develop

          Abilities of technological design

          Understandings about science and technology

     DEVELOPING STUDENT ABILITIES AND UNDERSTANDING

     This standard has two equally important parts developing students' abilities of technological
     design and developing students' understanding about science and technology. Although these
     are science education standards, the relationship between science and technology is so close
     that any presentation of science without developing an understanding of technology would
     portray an inaccurate picture of science.

     In the course of solving any problem where students try to meet certain criteria within
     constraints, they will find that the ideas and methods of science that they know, or can learn,
     can be powerful aids. Students also find that they need to call on other sources of knowledge
     and skill, such as cost, risk, and benefit analysis, and aspects of critical thinking and creativity.
     Learning experiences associated with this standard should include examples of technological
     achievement in which science has played a part and examples where technological advances
     contributed directly to scientific progress.

 The whole New Jersey study was centered around the technological aspect.  The use of the web and various computer modeling are examples of the technology involved in this project.
 

REFERENCES
 
Stankorb,Sheri., Stapp,William, and Walls, Arjen  Environmental Education for Empowerment. Kindall/Hunt Publishing Company,  Dubuque:1966

Introducing the National Science Education Standards, National Research Council,  Washington D.C.
 
 Model- It
Hi-C Research Group
Elliot Soloway, Dir
Reagents of the University of Michigan
Copyright 1995

 For Outreach see each web page of the team members listed below.

 
 FOR INDIVIDUAL MODELS USING THE DATA COLLECTED, CLICK ON THE   MODELS LISTED BELOW:

Annie Goldsmith-Wells, Stellar Model of the Nitrate Loading of Streams
Nancy Vawter, Nitrate Loading of Streams Using the River Model Computer Model
Helen Ballard and Gloria Stallworth, Classroom Activity