The Tree of Life
Using Four Techniques for Sampling
Arthropod Diversity On and Around a Tree

Berlese Funnel

Anthropod Collecting

Pittfall Trapping

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Activity: Pitfall Trapping

Purpose: To catch animals that crawl through the leaf litter.  These traps are usually left for several days allowing for the capture of nocturnal animals.

Materials (per trap):

  • 2 plastic disposable drinking cups
  • Piece of thin wood or particle board, approximately 12 cm x 12 cm
  • Small amount of propylene glycol (“safe” antifreeze), about 25 ml
  • Trowel or bulb planter

Procedure:

  1. Remove leaf litter from a small area.  Dig a hole just big enough for the cups, one nested inside of the other.  Leave the top edge of the cups level with the soil.
  2. Pour about 2 cm of propylene glycol into the cup.
  3. Lay the piece of wood over the cup, with sticks, rocks, or nails under the corners to hold this cover up about ¼" above the cup. (The purpose of the cover is to keep small mammals out of your traps.) Put a stick or stone on top of the cover to hold it in place. Redistribute the litter around the trap.
  4. Leave traps for a few days.
  5. Collect specimens from the traps by pouring the propylene glycol through a tea strainer or pick animals out with forceps.
  6. Store collected animals in alcohol.  Be sure to label your container.
  7. Identify and count animals.
  8. Return collected animals to clean alcohol for storage.

Placement and number of traps: One recommended protocol (Albert T. Finnamore Curator of Invertebrate Zoology at the Provincial Museum of Alberta) for obtaining 85% of the arthropods possible to trap in pitfalls is to set up 8 to 10 traps in a 10-meter circle.  Thus, one could place the tree in the center of the circle, with 8 to 10 traps spaced evenly in a circle five meters out from the tree. 
Tip : Do not thoroughly hide your traps.  You may not be able to find them again!  Either leave the cover free of litter or flag your traps.

Tip: Traditional antifreeze is good tasting to dogs and will kill them if they drink it.  Therefore, use the newer, safer antifreeze containing propylene glycol.  An alternative is to use a mixture of water, salt and detergent.   The salt will act as a preservative and the detergent (unscented) will break the surface tension so that the arthropods will sink and drown.

Tip: Cardboard will not work as a trap cover.  It will absorb moisture, come apart, and warp.

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