Abstract:
In this inquiry project, we enriched for certain protists – ciliates and  flagellates – which feed on bacteria and decomposing matter. A photographic film canister is used as a baiting trap. The trap is made by cutting a small window in the side of the canister and gluing nylon material to the edges of the window to form a screen. Water based tuna and some other foods are used as enrichment media. Traps are placed in water and checked after a day or more. They should contain a dense population of protists, which feed on bacteria that in turn feed on decomposing tuna fish. Natural pond water may provide a good source for enrichment of ciliates and flagellates.  A sample from the pond can be taken from the pond and used in the classroom.  If traps need to remain longer than one or two days, it is suggested that the aquatic environments be aerated.