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Sites Samples |
Results Day 2 |
Results Day 2 (40x) |
Results Day 4 |
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Plate 1. innoculated with sand |
Fungal strands, no amoebae |
Nematodes present |
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Plate 2. Pine needles |
Fungal hyphae and Amoebae |
Several nematodes present and a variety of protozoa, including ameoba and ciliates |
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Plate 3. Decayed log |
Amoebae appear yellowish in color , with a clear spot which could be the nucleus or vacuole. Fungi present |
Amoeba and subcolonies of varied bacteria. |
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Plate 5: Tree knee |
Amoebae present |
Nematodes, fungi and amoeba |
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Plate 6: Decomposing squirrel |
No amoeba |
Amoeba and fungi |
Observations were made after one week. Some plates had dried out therefore these results are not included in the above table. Some noteworthy observations are as follows: Plate 2 had clusters of nematodes that appeared to have reproduced. Plate 6 had excessive fungal growth demonstrating sporulation. There were numerous subcolonies of the Enterobacter aerogenes in all the plates.
Part II Results:
| Site | After 72 hours |
| Site 1: | Several medium white colonies with yellow centers and slightly scalloped edges. Small off white colonies with smooth edges. |
| Site 2: | Several small white smooth edged colonies. Numerous medium yellow scalloped edged colonies. Also large white colonies with deep scalloped edges. |
| Site 3: | A few medium yellowcolonies with smooth edges. A few small white colonies. Large white colonies with yellow centers and filamentous edges. |
| Site 4: | A few small yellow colonies with scalloped edges. Medium white colonies with darker centers. A few small gray colonies. A few large white colonies with smooth edges. A few small golden colonies with smooth edges. |
| Site 5: | Only large white colonies. |
| Site 6: | Densely packed tiny yellow and white colonies. Both had a fuzzy, beady appearance |
Analysis:
Based on the results of parts I and II of this investigation we interprete
that sites 2 and 4 contain the greatest variety of microorganisms;
ameoba, nematodes, ciliates, and fungi. Sites 2 and 4 also contained the
greatest number and variety of bacterial colonies (identified by morphology
of colonies only). Site 1, our control, revealed no ameoba and little
diversity in bacterial growth. Sites 3 and 5 showed intermediate
microbial life and bacterial growth.
Conclusion:
Based on our investigation we conclude that this procedure provides
for the isolation of ameoba as well as other microorganisms from soil.
| Introduction | Procedure | Main Page |
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| The
Woodrow Wilson National Fellowship Foundation
CN 5281, Princeton NJ 08543-5281 - Tel:(609)452-7007 - Fax:(609)452-0066 Technical contact: lpt@woodrow.org |