Sites Samples

Results Day 2

Results  Day 2  (40x)

Results Day 4

Plate 1. innoculated with sand

Fungal strands, no amoebae

Nematodes present

Plate 2.  Pine needles

Fungal hyphae and Amoebae

Several nematodes present and a variety of protozoa, including ameoba and ciliates

Plate 3.  Decayed log

Amoebae appear yellowish in color , with a clear spot which could be the nucleus or vacuole.  Fungi present

Amoeba and subcolonies of varied bacteria.

Plate 5: Tree knee

Amoebae present

Nematodes, fungi and amoeba

Plate 6: Decomposing squirrel

No amoeba

Amoeba and fungi

Observations were made after one week.  Some plates had dried out therefore these results are not included in the above table.  Some noteworthy observations are as follows: Plate 2 had clusters of nematodes that appeared to have reproduced. Plate 6 had excessive fungal growth demonstrating sporulation.  There were numerous subcolonies of the Enterobacter aerogenes in all the plates.

Part II Results:
 

Site After 72 hours
Site 1: Several medium white colonies with yellow centers and slightly scalloped edges.  Small off white colonies with smooth edges. 
Site 2: Several small white smooth edged colonies.  Numerous medium yellow scalloped edged colonies.  Also large white colonies with  deep scalloped edges. 
Site 3: A few medium yellowcolonies with smooth edges. A few small white colonies. Large white colonies with yellow centers and filamentous edges.
Site 4: A few small yellow colonies with scalloped edges. Medium white colonies with darker centers.  A few small gray colonies.  A few large white colonies with smooth edges.  A few small golden colonies with smooth edges.
Site 5: Only large white colonies.
Site 6: Densely packed tiny yellow and white colonies. Both had a fuzzy, beady appearance

 

Analysis:
Based on the results of parts I and II of this investigation we interprete that sites 2 and 4 contain the greatest variety of microorganisms;  ameoba, nematodes, ciliates, and fungi. Sites 2 and 4 also contained the greatest number and variety of bacterial colonies (identified by morphology of colonies only).  Site 1, our control, revealed no ameoba and little diversity in bacterial growth.  Sites 3 and 5 showed intermediate microbial life and bacterial growth.

Conclusion:
Based on our investigation we conclude that this procedure provides for the isolation of ameoba as well as other microorganisms from soil.
 
 

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