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Summary/Abstract
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Instructor's Objectives
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Target Audience
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Notes to Teacher
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Materials
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Background (prior knowledge or vocabulary
necessary to complete activity)
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Student Lab
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Evaluation/Assessment
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Extensions
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References
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Additional Links
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Summary/Abstract
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This activity is designed to show the effect of gravity on the growth of
plant roots. It is also designed to demonstrate the role that the growth
hormone auxin plays in the direction of root growth. In this lab, students
will place pinto beans on a turn table, and rotate the beans continuously
for five days. This continuous spinning will create a simulated field of
gravity on the beans.
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Based on the students' previous knowledge of the scienctific method, they
will hypothesize of the effect that this simulated field of gravity will
have on the seeds, specifically the roots that will grow. They will also
set up the experiment to test their hypothesis, gather results, analyze
their results and conclude whether or not their hypothesis was correct.
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Instructor's Objectives
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After completing this lab activity, the students will be able to:
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define the term tropism.
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name three different types of tropisms exihibited by plants.
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explain the role of auxin in plant root growth.
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evaluate the importance of gravity in root growth.
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form hypotheses and set up an experiment to test their hypotheses.
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Target Audience
Biology: grades 9 - 12
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Notes to Teacher
This activity calls for a turntable (record player) to rotate the
beans as they are germinating. If a turntable is not available, any device
which will spin the beans at about the same speed will work just as well.
The protocol also says that the beans must stand for three days in the
aluminum foil before spinning the beans. Try to have the students prepare
the aluminum foil packets on a Friday, therefore the beans can stand undisturbed
over the weekend and be ready to go on Monday morning. Also, if only one
turntable is available, this could be done as a demonstration by the teacher.
If pinto beans are not available, raddish seeds, corn, and peas could be
used instead.
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Materials
16 pinto beans
glass or beaker
paper towels
record player
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Background
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Student Lab
Teacher preparation:
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Place the beans in a beaker or glass filled with water (tap water is fine).
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Allow the beans to soak in the water in the refridgerator overnight.
Student Lab Procedure:
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Tear four pieces of aluminum foil that are about one-foot (30 cm) square.
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Moisten a paper towel with water (the paper towel should be damp, not dripping
wet!)
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Place the paper towel in the middle of each piece of foil.
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Get four beans which have been soaked overnight by the teacher, and place
them in the center of each paper towel
(See
Figure B below).
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Fold the aluminum foil around the paper towel and close each end of the
foil, forming a pouch with the beans inside (You might want to place a
piece of masking tape on your packet and write your name on it, since there
will be more than one packet on the turntable). See Figure C below.
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Let the bean packets stand undisturbed for 3 days.
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Place the seed packets, evenly spaced, on the turntable.
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Turn the record player on and place it on 75 rpm.
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Allow the record player to rotate continuously for 5 days.
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After 5 days, turn off the record player, obtain your seed packets.
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Open your packet and make observation about the direction of root growth.
See Figure D below.
(copied with permission from Biology for Every Kid by Janice Van
Cleave)
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Evaluation/Assessment
There are a couple of different ways that this activity can be assessed:
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Students can be assigned a journal which must be kept and updated throughout
the activity. This observation log can include their hypothesis which was
formed before the activity was started. Being good amateur scientists,
they might also include a detailed description of the actual lab set-up
and equipment used. It can also include all of the post lab observations
that they make concerning the seeds. Students would also include their
rationale of whether or not their hypothesis was correct. A rubric could
be used to grade this form of assessment, such as: 20 pts for their hypothesis;
20 pts for a detailed description of the set-up; 40 pts. for detailed observations
of the beans after 5 days of spinning - this would include drawings of
the seeds after the experiment; 10 pts. for student rationale defending
or rejecting their hypothesis; 10 pts for overall neatness of the journal.
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A post lab quiz could be given to assess student achievement. The following
questions could be asked:
1. Name 3 other tropisms that plants exhibit in response to their environment.
Also, name what each
tropism is in response to (e.g., thigmotropism - response to touch).
2. What effect, if any, did spinning have on the beans?
3. Describe the role of the plant hormone auxin in the growth of a plant.
4. What do you think would happen if you reversed the direction that the
beans were spinning?
Explain your answer.
5. Design an experiment that would test one of the other tropisms that
you named in question #1.
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Since one of the steps of the scientific method is communicating, the students
could present their results to the entire class. In their presentations,
they must state their original hypotheses, present their results using
visual aids (posters, overheads, graphs, etc.), and relate their results
to the overall theme of plant movement. Again, a rubric could be used to
grade this method of assessment: 20 pts for statement of original hypothesis,
30 pts for visual aids, 30 pts for statement of results, and 20 pts for
overall effectiveness of presentation (clarity, smoothness, etc.).
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Extensions
This lab activity can modified into an inquiry based activity in which
the students must design a lab to test their hypotheses. In order to do
this, the teacher can discuss the role of plant hormones, such as auxin,
with the students. At that point the teacher could ask the class what else
they want to know about tropisms and write the student generated questions
on the board. The students would then form hypotheses about whichever tropism
they wanted to investigate. Give the students the presoaked beans, have
them design an experiment to test their hypothesis, and then actually set-up
and run the experiment. Students would gather data throughout their experiment
and present their results to the class.
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References
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Additional Links
http://biocomp.arc.nasa.gov/people/jeff/.index.html
- Current research on gravitropism by Jeffrey Smith, a Ph. D. at NASA.
http://trc.ucdavis.edu/Coursepages/EXCITES/ls.html
- Another gravitropism experiment using Wisconsin fast plants.
http://www.aspp.org/education/labexer.htm
- Lab activity using corn seeds to demonstrate gravitropism.
http://eruditio.asu.edu/~smckeeve/scilinkbio.html#Plants
- Other plant links and activities using plants
http://www2.ncsu.edu/unity/lockers/project/ncsu-nscort/public/outreach/teach/outline.html
- Home page of the NASA Specialized Center of Research and Training in
gravitational biology at NC State University.
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