Student Lab Sheet                        Name_____________________ date_____________
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Introduction 
 
 

Biology Talk Show        Guest:  Dr. Ernest Everett Just
 
    (Teacher can serve as talk show host or two students do a dialogue to introduce lesson.)

Host:       Today on our show I am pleased to welcome Dr. Ernest Everett Just, a pioneer in
                marine embryology.  He has overcome great adversities in his personal and
                professional life and has made extraordinary accomplishments in his field.
 
                Welcome, Dr. Just.  It is a pleasure to have you here at our school.  Everyone here is
                anxious to get to know you.  First, tell us about your background.

Just:        I had a difficult youth.  My father was an alcoholic and could never keep a steady job.
                He died when I was very young.  My older brother and older sister died also due to
                poor health.  It was my mother who was the strength for myself, my younger brother,
                and my younger sister.  When my dad died we were extremely poor.  Thanks to my
                mother, who was incredibly resourceful, we survived.

Host:      How did your mom keep the family together?

Just:        She was a dedicated teacher, and she made huge sacrifices for me to continue
                my education. Actually,  she was the one who encouraged me to
                attend Kimball Hall Academy in New Hampshire.

Host:        I understand it was a struggle to attend.

Just:        I earned a scholarship, however, I didn't have enough money for the fare
               to New Hampshire.  I decided to find work along the way from Charleston.
               To pay my ship fare from Charleston to New York, I worked on the ship.
               In New York I worked as a cook long enough to pay my fare to New
               Hampshire.  It wasn't easy, but I believe you can accomplish anything
               if you really want it.

Host:       You earned awards in botany, sociology, and history at Kimball.
                Your mother must have been very proud.

Just:         I was the only African American in an all white school.  I was
                eager to learn and persistent in my efforts to accomplish great
                things.  Unfortunately, my mother never saw me graduate with
                high honors.  She died and never knew I earned a scholarship
                to Dartmouth.

Host:       You must have been a stellar student at Dartmouth.

Just:        Actually, no.  Not in the beginning.  Like many freshmen I didn't
                work hard as I could have.  I turned it around later.  One professor,
                Dr. Patten, encouraged and got me excited in the sciences.  I chose
                zoology as my major.

Host:       Was that a good field to enter?

Just:        Not for a black man or woman in the early 1900's.  There
                weren't many choices for a minority in the sciences.  Actually,
                my only option was to teach in a black community.  I began
                teaching at Howard University in Washington, D.C.

Host:        If there weren't many opportunities for you, how did you end up
                as a biologist in a primarily white research facility--the Marine
                Biological Laboratory in Woods Hole, Massachusetts.

Just:         I worked my way up from a research assistant--taking demanding
                classes and learning proper research techniques.  I was persistent
                and worked hard because I was dedicated to my field.  I gained
                respect because of my research.

Host:       Describe briefly some of your research.

Just:        I worked on fertilization experiments with marine animals such as
                sea urchins and marine annelids.

Host:        I understand many embryologists sought your advice on designing
                experiments and handling specimens.

Just:        Yes, I pay particular attention to this.  I was very meticulous about my
                research.  You cannot conduct good research with damaged specimens.

Host:        Did the other researchers accept you in their social gatherings?

Just:         I was accepted by a small group of white biologists.  Others were
                prejudiced, which made it difficult to join them in a social setting.
                Actually, some white researchers ignored my work.  That crushed
                my spirit.  I felt hurt and frustrated.

Host:        Is that why you left Woods Hole to do research in Europe?

Just:        Yes, there were racial tensions at Woods Hole.  The institutes in
                Naples, Paris, and Berlin felt like a community. It is a shame that
                I did not feel welcome in my own home country.  I was respected
                and accepted in Europe.

Host:       Did you eventually leave Europe because of the war?

Just:        Yes, the Germans were invading Paris in the summer of 1940. During
                that time I was jailed.  I was fortunate that the United States government
                could get me out of jail and take me back home.

Host:       Since you have faced many obstacles, how would you encourage
                someone in pursuit of their goals in the face of adversity?

Just:        I would tell him or her to be determined.  Have self confidence.  Work
                hard at what you love.

        (Audience claps)

Host:        I understand you have an activity we can do in the classroom.

Just:        Yes, this lab works with Nereis worms.  We will try to induce
                parthenogenesis in Nereis worm eggs.  All students should read
                the entire lab procedure before attaining materials to begin the lab.
                Keep the specimens covered to prevent them from drying out.

Equipment
        Each team of 2-3 students will need:
            *4 female Nereis worms(kept covered in a small petri dish)
                (or female sea urchins)

            *Seawater or Instant Ocean water at room temperature(18-20oC)-
                enough for rinsing worms and to fill 3 small glass bowls.

            *1 piece of filter paper
            *Large test tube with sea water heated to 30-33oC
            *Another large test tube
            *3 small glass bowls
            *Watch/clock
            *pipette(glass or plastic)
            *thermometer

 

Procedure
            1)  Rinse at least 2 female worms with sea water at room temperature
                       and dry on filter paper.

            2)  Transfer the other 2 worms in the sea water to a test tube to use as
                        your control.

            3)  Place female worms in test tube containing sea water that is warmed
                        to 30-33oC.  Watch for the shedding of eggs and the extrusion
                        of jelly.

            4)  After five minutes pipette a drop of eggs to a small glass bowl
                      containing sea water at room temperature(18-20oC).  Record
                      observations in table.  Keep track of how long it takes for any
                      changes to take place.

            5)    Wait another 5 minutes and pipette another drop of eggs in
                    a second bowl containing room temperature sea water.  Record
                    observations.

            6)     After another five minutes repeat step #3.  Record observations.

Observations   
        Describe what happened to the eggs in each of the three bowls and record the
        time it took for changes to take place.  Compare results to the control.
Observations (minutes for changes)
Control test tube           min. 
Bowl #1 (after 5 minutes)           min.
Bowl #2 (after 10 minutes)           min.
Bowl #3 (after 15 minutes)           min.
   Conclusions  
 

1)  What variable was altered in the experiment to induce parthenogenesis in the
        eggs?  How do you know parthenogenesis took place?
 
 

2)  What do you think was my hypothesis on how to induce parthenogenesis in
        these eggs?
 

3)  Did you induce parthenogenesis in all 3 bowls?  Note any similarities and
     differences in changes.
 
 

4)  How could you modify my experiment to test your own hypothesis?  What would
      your hypothesis be?
 
 
 
 
 
 

 
 

(Note:  Ernest Just died at the age of 58 of cancer, just a year after returning to the United
States.)

*for suggestions on how to teach multicultural science