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Biotechnology

1993 Biology Institute Participants
1993 Biology Institute Faculty

Part I. General Overview

Overview and History of Biotechnology
Students use a timeline of key events in biotechnology to discuss modern trends in science
Future of Biotechnology
Background information about biotechnology

Part II. Background Information

Basic Biotechnology for Beginning Teachers
Hints, tips, suggestions, resources, suppliers, and words of wisdom to make beginning biotechnology in your classroom more user-friendly
Overview of DNA Manipulation
Explanations of the cloning process
Growth Regulation of Cells
Slides from faculty lectures

Part III. Laboratory Activities

Isolation of DNA from Onion
DNA without tears!
The Spread of AIDS
A simple experiment using phenopthalein and tubes of water to demonstrate the epidemiology of HIV-1
Separation of a Starch-Glucose Mixture Using Gel Filtration
Fun with Sephadex G-25 columns!
The Cookbook Translator
Students "translate" a lima bean DNA extraction procedure
Protein Assays: Bio Rad and Page Gels
Learn how to use the spectrophotometer and micropipette
Phenotypic and Genotypic Changes in Bacteria
Bacterial tranformations using antibiotic resistant plasmids
Rainbow Electrophoresis
Introduction to electrophoresis
Using Yeast as an Ultraviolet Light Measurement Tool
Measuring UV using yeast cells...adapted from KSU's Yeast Project
Karyotype Alternatives
Two activities for karyotyping: a paper cut out method and a lab usinghuman cells
Cloning of Plasmid and Spinach DNA
Procedure to ligate fragments of genomic DNA from spinach into a vector plasmid; this recombinant DNA is then used to transform Escherichia coli cells.
The Cloning of Plasmid and Spinach DNA
Inserting a spinach genomic clone into a plasmid
Chick Embryology
A couple of easy techniques which can be used to observe the development of a chick embryo
Culturing Plants from Embryonic Plant Tissue
The purpose of this lab is to grow plants in tissue cultures and to see what effect the different concentrations of hormones have on embryodevelopment
Protoplast Production
This activity allows students to strip away the cell walls of plant cells (using enzymes) and then observe the resulting spherical protoplasts
Comparison of Four-, Six-, and Eight-bp Cuts in Calf Thymus DNA
Using restriction enzymes to digest thymus DNA
Demonstrating an Epidemic
An experiment using bacterial cultures to simulate a small scale "epidemic"
Cell Transformation in Tobacco Leaf Disks
A relatively simple plant transformation experiment
Active Ingredient Screening Test for Plants
Students simulate tests done in the field to determine the active ingredients in plants
Ecological Bioassay of Copper Sulfate in Daphnia Magna
This ecological laboratory uses the biotechnological tool of the bioassay to demonstrate the effects of toxic substances on living organisms

Part IV. Computer Applications

Use of HyperCard and Laserdisc to Demonstrate Reproductive Technologies
Biotechnology Topics: A Selected List of Related Laserdisc Frame Numbers
A Multimedia Presentation Using Linkway Live: A Review of "The Cell"

Part V. Classroom Activities

Who Done It?
DNA Forensics simulation
Biotechnology in Nature
Creative Expressions -- Protein Synthesis
Creating three-dimensional models to explain translation
Genetic Control of Pattern Formation
Highlights from a lecture by Dr. Eric Wieschaus
Molecular Biology of Cancer
Highlights Of Lecture By Arnold Levine, Princeton University
Cracking the Code/Cloning Paper Plasmid
Activities that provide a review of the "genetic code" and the role it plays in our life and to help students see how genes may be manipulated for genetic research
Teaching Biotechnology by Analogies and Models
Simple analogies to enhance student understanding of biotech techniques and principles
Enzyme Function Analogies
An alternative to memorization!
"Biotechnology" Recipe Analogies
Electrophoresis Analogy
It's simple....and it works!
Gene/Chromosome - Gene/DNA Relationship Analogies
Virus Analog
Serial Dilutions Made Easy
1/10...1/100...1/1000
Creating New Forms of Life
Students are asked to create their own "life form"
Desktop Electrophoresis Lab: Moving Molecules
Teaching Biotechnology with Models
Students "build" a cell and a plasmid using household items to aid in the understanding of bacterial transformation
Mutations--Preexisting or Acquired?
Observations of phage-infected bacterial cells serve as the basis for class discussion
Amazon Adventure
Student role-play to uncover the medicinal properties of an imaginary Amazonian plant
Recombinant DNA to Protect Crops
An article to demonstrate how worthwhile gene manipulation in plants can be
Construction of a Hemoglobin Gene

Part VI. Other Teacher Presentations at the Institute

Creative Ways to Teach Evolutionary Concepts
Laugh a little....learn a lot!
A Spool of Thread
A demonstration to show the relationship between DNA and a chromosome using a large spool of light colored thread
Green Jeans Horticulture
This course was devised to accommodate students of average and below-average academic ability and provide them with practical horticultural experiences
The Spanish Omelet
The quest for an omlet recipe is used to explain transcription and tranlation!

Part VII. Appendices

Biotechnology: Safety in the Classroom
Saftey Guide taken from NABT's "Working with DNA & Bacteria in Precollege Science Classrooms"
Some Biotechnology Safety Precautions
Classroom safety information
Student Safety Checklist
Preparing students for lab experiments
Spreading a Yeast or Bacterial Lawn
Creating beautiful lawns that don't need mowing!
Streaking for Isolation
The easy way to culture individual colonies of bacteria
Surface Sterilization of Biological Materials
Bugs-B-Gone!
Materials Catalogs
A list of biotech material suppliers
Biotechnology Bibliography
Resources for further reading on biotechnology

Acknowledgments

[NSF Logo] This program was supported by the National Science Foundation under Grant TPE-9155255. [AE Logo] These materials were converted to HTML by Access Excellence, a program of Genentech, Inc.
Opinions expressed are those of the authors and not necessarily those of the contributing organizations.

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